Bioactivation with laccase for the removal of pharmaceutical contaminants in wastewater: laboratory level, 2025
DOI:
https://doi.org/10.55873/rba.v4i2.390Keywords:
bioactivation, pharmaceutical, immobilizationAbstract
The present study contributes to Sustainable Development Goal (SDG) 6: “Ensure availability and sustainable management of water and sanitation for all”, by investigating the efficiency of immobilized laccase in the removal of pharmaceutical contaminants in wastewater, with objectives to analyze bioactivation with laccase, determine the optimal treatment conditions and evaluate the efficiency of laccase immobilization, through a basic, quantitative and pure experimental study, with a study population of 24 liters of wastewater with pharmaceutical contaminants elaborated at laboratory level, obtaining results that the application of laccase immobilized on silica support material obtained from cucumber peel (biochar) is an effective technology for the removal of these contaminants, reaching reductions of up to 93.8%, suggesting that immobilized laccase is a promising technology for the removal of pharmaceutical contaminants in wastewater, contributing to sustainable water management.
References
Agustin, Melissa B., et al. Laccase as a tool in building advanced lignin‐based materials. ChemSusChem, 2021, vol. 14, no 21, p. 4615-4635. https://doi.org/10.1002/cssc.202101169
Ahmad, Imran, et al. Catalytic insights into laccase for sustainable remediation of multifaceted pharmaceutically active micropollutants from water matrices: A state-of-art review. Journal of Water Process Engineering, 2025, vol. 70, p. 106901. https://doi.org/10.1016/j.jwpe.2024.106901
Al-Sareji, Osamah J., et al. Efficient removal of pharmaceutical contaminants from water and wastewater using immobilized laccase on activated carbon derived from pomegranate peels. Scientific Reports, 2023, vol. 13, no 1, p. 11933. https://doi.org/10.1038/s41598-023-38821-3
Almufarij, Rasmiah S.; Abdulkhair, Babiker Y.; Salih, M. Fast-simplistic fabrication of MoO3@ Al2O3-MgO triple nanocomposites for efficient elimination of pharmaceutical contaminants. Results in Chemistry, 2024, vol. 7, p. 101281. https://doi.org/10.1016/j.rechem.2023.101281
Alokpa, Komla; Vaidyanathan, Vinoth Kumar; Cabana, Hubert. Immobilization of laccase for industrial wastewater treatment: Current challenges and future perspectives. En Laccase and Polyphenol Oxidase. Academic Press, 2025. p. 187-246. https://doi.org/10.1016/B978-0-443-13301-5.00008-1
Alokpa, Komla; Lafortune, François; Cabana, Hubert. Application of laccase and hydrolases for trace organic contaminants removal from contaminated water. Environmental Advances, 2022, vol. 8, p. 100243. https://doi.org/10.1016/j.envadv.2022.100243
Aza, Pablo; Camarero, Susana. Fungal laccases: Fundamentals, engineering and classification update. Biomolecules, 2023, vol. 13, no 12, p. 1716. https://doi.org/10.3390/biom13121716
Bai, Yongsheng, et al. Characterization of plant laccase genes and their functions. Gene, 2023, vol. 852, p. 147060. https://doi.org/10.1016/j.gene.2022.147060
Barber-Zucker, Shiran, et al. Designed high-redox potential laccases exhibit high functional diversity. ACS catalysis, 2022, vol. 12, no 21, p. 13164-13173. https://doi.org/10.1021/acscatal.2c03006
Boni, M. R., et al. A novel treatment for Cd-contaminated solution through adsorption on beech charcoal: The effect of bioactivation. Desalination and water treatment, 2018, vol. 127, p. 104-110. https://doi.org/10.5004/dwt.2018.22664
Chandra, Muni Ramanna Gari Subhosh; MADAKKA, Mekapogu. Comparative biochemistry and kinetics of microbial lignocellulolytic enzymes. En Recent developments in applied microbiology and biochemistry. Academic Press, 2019. p. 147-159. https://doi.org/10.1016/B978-0-12-816328-3.00011-8
Ghose, Anamika, et al. Micropollutants (ciprofloxacin and norfloxacin) remediation from wastewater through laccase derived from spent mushroom waste: Fate, toxicity, and degradation. Journal of Environmental Management, 2024, vol. 366, p. 121857. https://doi.org/10.1016/j.jenvman.2024.121857
Gome, A.; Upadhyay, K. Removal of persistent chemical oxygen demand from pharmaceutical wastewater by ozonation at different pH. International Journal of Environmental Science and Technology, 2023, vol. 20, no 2, p. 2087-2098. https://doi.org/10.1007/s13762-022-03915-4
González Martínez, Constancio. La Investigación básica. La investigación en ciencias fisiológicas: bioquímica, biología molecular y fisiología. Cuestiones previas. Educación médica, 2004, vol. 7, p. 41-50. ISSN 1575-1813
Gu, Haolun, et al. Bioinspired bimetallic metal–organic framework nanozyme with laccase-mimicking activity for detection and removal of phenolic contaminants. Microchemical Journal, 2024, vol. 201, p. 110568. https://doi.org/10.1016/j.microc.2024.110568
Han, Meina, et al. Cytoprotective alginate microcapsule serves as a shield for microalgal encapsulation defensing sulfamethoxazole threats and safeguarding nutrient recovery. Journal of Hazardous Materials, 2024, vol. 465, p. 133454. https://doi.org/10.1016/j.jhazmat.2024.133454
Hassan, Ayat, et al. Bio-activation and mathematical modeling of ZIF-L encapsulated with laccase for enhanced ibuprofen removal from wastewater. Cleaner Engineering and Technology, 2024, p. 100875. https://doi.org/10.1016/j.clet.2024.100875
Hashempour, Yalda, et al. Co-immobilization of laccase and zinc oxide nanoparticles onto bacterial cellulose to achieve synergistic effect of photo and enzymatic catalysis for biodegradation of favipiravir. International Journal of Biological Macromolecules, 2024, p. 139288. https://doi.org/10.1016/j.ijbiomac.2024.139288
Hu, Yingli, et al. Lipase immobilization on macroporous ZIF-8 for enhanced enzymatic biodiesel production. ACS omega, 2021, vol. 6, no 3, p. 2143-2148. https://doi.org/10.1021/acsomega.0c05225
Hu, Xiaolei, et al. MIL-100-Fe self-assembled cellulose nanofibers sponge for Diclofenac cascade encapsulation. Carbohydrate Polymers, 2024, p. 123182. https://doi.org/10.1016/j.carbpol.2024.123182
Iñiguez, Pedro Cadena, et al. Métodos cuantitativos, métodos cualitativos o su combinación en la investigación: un acercamiento en las ciencias sociales. Revista Mexicana de Ciencias Agrícolas, 2017, vol. 8, no 7, p. 1603-1617. ISSN 2007-0934
Janusz, Grzegorz, et al. Laccase properties, physiological functions, and evolution. International journal of molecular sciences, 2020, vol. 21, no 3, p. 966. https://doi.org/10.3390/ijms21030966
Kinga Skalska-Tuomi, et al. Efficient removal of pharmaceuticals from wastewater: Comparative study of three advanced oxidation processes. Volume 375, February 2025, 124276. https://doi.org/10.1016/j.jenvman.2025.124276
Kumar, Ramesh, et al. Advancing pharmaceutical wastewater treatment: A comprehensive review on application of catalytic membrane reactor-based hybrid approaches. Journal of Water Process Engineering, 2024, vol. 58, p. 104838. https://doi.org/10.1016/j.jwpe.2024.104838
Majumder, Abhradeep; GUPTA, Bramha; GUPTA, Ashok Kumar. Pharmaceutically active compounds in aqueous environment: A status, toxicity and insights of remediation. Environmental research, 2019, vol. 176, p. 108542. https://doi.org/10.1016/j.envres.2019.108542
Mehra, Rukmankesh, et al. A structural-chemical explanation of fungal laccase activity. Scientific Reports, 2018, vol. 8, no 1, p. 17285. https://doi.org/10.1038/s41598-018-35633-8
Nguyen, Minh-Ky, et al. Occurrence and fate of pharmaceutical pollutants in wastewater: Insights on ecotoxicity, health risk, and state–of–the-art removal. Chemosphere, 2024, p. 141678. https://doi.org/10.1016/j.chemosphere.2024.141678
Nguyen D. et al. Microplastics and pharmaceuticals from water and wastewater: Occurrence, impacts, and membrane bioreactor-based removal. Separation and Purification Technology. 2025, p. 131489. https://doi.org/10.1016/j.seppur.2025.131489
Peng, Quancai, et al. Pharmaceutically active compounds (PhACs) in surface sediments of the Jiaozhou Bay, north China. Environmental Pollution, 2020, vol. 266, p. 115245. https://doi.org/10.1016/j.envpol.2020.115245
Peña-Guzmán, Carlos, et al. Emerging pollutants in the urban water cycle in Latin America: A review of the current literature. Journal of environmental management, 2019, vol. 237, p. 408-423. https://doi.org/10.1016/j.jenvman.2019.02.100
Pinheiro, Bruna B., et al. Genipin and glutaraldehyde based laccase two-layers immobilization with improved properties: New biocatalysts with high potential for enzymatic removal of trace organic contaminants. Enzyme and Microbial Technology, 2023, vol. 169, p. 110261. https://doi.org/10.1016/j.enzmictec.2023.110261
Salgado Costa, Carolina, et al. Linking environmental exposure and effects of pharmaceuticals on aquatic biota: state of knowledge in Latin America. 2023. https://doi.org/10.20517/wecn.2023.08
Singh, Anil Kumar, et al. Laccase-based biocatalytic systems application in sustainable degradation of pharmaceutically active contaminants. Journal of Hazardous Materials, 2024, p. 136803. https://doi.org/10.1016/j.jhazmat.2024.136803
Valdez-Carrillo, Melissa, et al. Pharmaceuticals as emerging contaminants in the aquatic environment of Latin America: a review. Environmental Science and Pollution Research, 2020, vol. 27, p. 44863-44891. https://doi.org/10.1007/s11356-020-10842-9
Vallejo Maite. El diseño de investigación: una breve revisión metodológica. Archivos de cardiología de México, 2002, vol. 72, no 1, p. 8-12. ISSN 1405-9940
Wang, Hao, et al. Laccase immobilization and its degradation of emerging pollutants: A comprehensive review. Journal of Environmental Management, 2024, vol. 359, p. 120984. https://doi.org/10.1016/j.jenvman.2024.120984
Wang, Zhaobo, et al. Study on improving the stability of adsorption-encapsulation immobilized Laccase@ ZIF-67. Biotechnology Reports, 2020, vol. 28, p. e00553. https://doi.org/10.1016/j.btre.2020.e00553
Yang, Xue, et al. Encapsulated laccase in bimetallic Cu/Zn ZIFs as stable and reusable biocatalyst for decolorization of dye wastewater. International Journal of Biological Macromolecules, 2023, vol. 233, p. 123410. https://doi.org/10.1016/j.ijbiomac.2023.123410
Zhu, Mengzhen, et al. The electron shuttle of aloe-emodin promotes the Cu-FeOOH solid solution photocatalytic membrane to activate hydrogen peroxide for the degradation of tannic in traditional Chinese medicine wastewater. Applied Catalysis B: Environment and Energy, 2025, vol. 361, p. 124566. https://doi.org/10.1016/j.apcatb.2024.124566
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Grecia del Pilar Chuquival-Fababa, Llelin Fasabi-Pisco
This work is licensed under a Creative Commons Attribution 4.0 International License.
